ACTIVATION OF NEUTROPHILS NADPH OXIDASE BY PMA - CYTOSOL ACTIVITY IS TRANSLOCATED IN PHORBOL-PRIMED NEUTROPHILS

1. Translocation of cytosol activity in phorbol-primed neutrophils was studied. 2. Prior exposure of PMA or FMLP could potentiate the oxidat ive response by subsequent heterogeneous stimulus, FMLP or PMA. 3. In FMLP-primed neutrophils, the cytosol had almost the same activity as r esting one and cytosol activity was not eluted from the membrane. 4. I n PMA-primed neutrophils, however, the cytosol had less activity and c ytosol activity was correspondingly eluted from the membrane. 5. These observations suggested that cytosol activity was translocated in PMA- primed cells.