CHARACTERIZATION OF PORCINE POLYMORPHIC MICROSATELLITE LOCI

Twenty-seven (CA)n and two (GA)n microsatellite clones were isolated o ut of a size-selected genomic pig library. These were sequenced and th e number of uninterrupted dinucleotides was found to range from 12 to 26. Flanking primers were chosen for 11 dinucleotide repeats and optim al conditions for polymerase chain reaction (PCR) amplifications were established. Different microsatellite loci were amplified simultaneous ly by combining primer sets. Related and unrelated pigs were screened for length polymorphisms of the different microsatellite loci. The pol ymorphic information content (PIC) of these loci ranged between 0-62 a nd 0.83. Segregation studies in pig reference families established Men delian inheritance. Locus S0022 was found to be X-linked.