IDENTIFICATION OF AN OPERON INVOLVED IN THE ASSIMILATORY NITRATE-REDUCING SYSTEM OF AZOTOBACTER-VINELANDII

Authors
RAMOS F BLANCO G GUTIERREZ JC LUQUE F TORTOLERO M
Citation
F. Ramos et al., IDENTIFICATION OF AN OPERON INVOLVED IN THE ASSIMILATORY NITRATE-REDUCING SYSTEM OF AZOTOBACTER-VINELANDII, Molecular microbiology, 8(6), 1993, pp. 1145-1153
Citations number
36
Categorie Soggetti
Biology,Microbiology
Journal title
Molecular microbiology → ACNP
ISSN journal
0950382X
Volume
8
Issue
6
Year of publication
1993
Pages
1145 - 1153
Database
ISI
SICI code
0950-382X(1993)8:6<1145:IOAOII>2.0.ZU;2-R
Abstract
A number of mutants lacking nitrate reductase (Nas-) or nitrite reduct ase (Nis-) activities have been isolated and characterized. An operon including two new genes (nasA and nasB) has been defined and cloned fr om an Azotobacter vinelandii gene bank. nasA encodes for nitrite reduc tase apoenzyme, whereas nasB is specific for nitrate reductase activit y. Nitrate reductase exerts a regulatory effect on nasAB.