SEQUENCE HOMOLOGY AND SIMILAR PERIODICITY OF 700 BP BETWEEN SATELLITEDNAS OF BUFFALO AND CATTLE

Buffalo genomic DNA digested with Pst I restriction endonuclease revea led 5 conspicuous bands of repetitive DNA fragments by horizontal agar ose-gel electrophoresis. The molecular sizes of the fragments were 0.5 ,0.7, 1.25, 1.35 and 2.1 kbs. Buffalo genomic DNA - Pst I digests hybr idized with cattle -Eco RI 1.4 kb repetitive DNA fragment even under h igh stringent condition, hence, revealed high degree of sequence homol ogy between satellites of buffalo and cattle. Southern hybridization p atter revealed bands of 0.69, 1.39, 2.11, 2.75 and 3.43 kbs and thus d emonstrated a periodicity of approximately 700 bp, similar to that obs erved in cattle. Agarose-gel electrophoresis and southern blot hybridi zation revealed sequence homology and similar periodicity of 700 bp be tween satellites of buffalo and cattle.