Jb. Lamture et Tg. Wensel, A NOVEL REAGENT FOR LABELING MACROMOLECULES WITH INTENSELY LUMINESCENT LANTHANIDE COMPLEXES, Tetrahedron letters, 34(26), 1993, pp. 4141-4144
A novel bifunctional chelator, 4-iodoacetamidodipicolinicacid, has bee
n synthesized in three steps starting with chelidamic acid, and used t
o prepare protein conjugates that form stable and intensely luminescen
t complexes with Tb(III) and Eu(III). The excited state lifetime of Tb
(III) complexes with this reagent was 1.25 ms, permitting its use for
ultrasensitive time-resolved luminescence assays.