CELLULASE FROM HUMICOLA SPP

The newly isolated Humicola strain secreted cellulases in the culture medium supplemented with 1% water hyacinth as carbon source. Of the ni trogen sources investigated, ammonium sulphate (0.5%) and yeast extrac t (4%) produced the highest amount of the enzyme complex. Maximal cell ulases were synthesized at pH 6.0 and temperature 35-degrees-C. The cu lture filtrate from this organism was used to study the saccharificati on of lignocellulosic wastes and adsorption onto avicel. About 48% sac charification was achieved with rice straw after a period of 24 h hydr olysis. The endoglucanase in the culture filtrate was adsorbed onto av icel to the extent of 13%.