DIFFERENCES IN HEMOLYTIC ACTION OF HG2-STRENGTH OF INCUBATING SOLUTIONS( IN RELATION TO ITS CONCENTRATION AND IONIC)

The haemolytic action of different concentrations of HgCl2 on rat red blood cells (RBC ) was studied in vitro. The concentrations of HgCl2 i n incubating media were 0.15, 0.25 and 0.50 mmol.l-1. The ionic streng th of the media varied from 0 to 154 mmol.l-1 NaCl. Isotonicity of sol utions was also compensated using isotonlc glucose in different concen trations (287-0 mmol.1-1). Osmolarity of solutions varied from 287 to 308 mOsm. Besides these solutions the haemolysis in Krebs-Ringer solut ion was also studied. Haemolysis was characterized with two maxima in all concentrations of Hg2+. The first maximum was observed at low toni c strength and the second one at high tonic strength. In relation to t he increased concentrations of Hg2+, the first maximum of haemolysis p rogressively declined towards the higher ionic strength. In the Krebs- Ringer solutions, the increased concentration of Hg2+ was followed by reduced haemolysis. The haemolytic concentration of 0.15 mmol.l-1 was found to be optimal.