H. Ishizaka et J. Uematsu, PRODUCTION OF PLANTS FROM POLLEN IN CYCLAMEN-PERSICUM MILL THROUGH ANTHER CULTURE, Ikushugaku Zasshi, 43(2), 1993, pp. 207-218
Anther culture response was examined in diploid (2n = 2x = 48) and tet
raploid (2n = 4x = 96) cultivars of C. persicum. Embryoids were produc
ed in N6, BS or MS medium containing 0.02 approximately 2 mg/l NAA and
6% to 12% sucrose under dark conditions although the optimum basic me
dium for embryo production was N6 or B5 and the optimum sucrose concen
tration for embryo production was 9%. The anthers containing early- an
d late-uninucleated pollen grains produced embryoids, while those cont
aining early-binucleated pollen grains were completely unresponsive. E
mbryoid production was inhibited by high temperature treatment, but pr
omoted by low temperature treatment at 5-degrees-C for 2 days. The emb
ryoids developed into plantlets by transplanting to the B5 medium cont
aining 3% sucrose. Chromosome observation of the mature plants derived
from these embryoids showed that haploid and diploid plants were obta
ined by anther culture of diploid cultivars, and that dihaploid and te
traploid plants were obtained by anther culture of tetraploid cultivar
s. Cytological observation of pollen grains during anther culture show
ed the presence of multicellular grains at various stage. These result
s suggest that plants obtained by anther culture of cyclamen originate
d from pollen grains.