FUNCTIONS OF INTERLEUKIN-8 ARE MEDIATED THROUGH THIOL GROUP(S) OF IL-8 RECEPTOR IN HUMAN POLYMORPHONUCLEAR NEUTROPHILS - EFFECTS OF 5,5'-DITHIO-BIS(2-NITROBENZOIC ACID) ON IL-8 RECEPTOR
S. Dutta et al., FUNCTIONS OF INTERLEUKIN-8 ARE MEDIATED THROUGH THIOL GROUP(S) OF IL-8 RECEPTOR IN HUMAN POLYMORPHONUCLEAR NEUTROPHILS - EFFECTS OF 5,5'-DITHIO-BIS(2-NITROBENZOIC ACID) ON IL-8 RECEPTOR, FEBS letters, 325(3), 1993, pp. 262-266
Interleukin-8, a neutrophil chemotactic agent causes excessive accumul
ation of the cells in a number of inflammatory diseases. The activity
has been shown to be mediated through a specific functional receptor p
resent on the surface of neutrophils. No information is available abou
t the amino acids constituting the IL-8 binding domain of the receptor
. Treatment of neutrophils with 5,5'-dithio-bis(2-nitrobenzoic acid),
a thiol-specific modifier, at the concentrations of 0.4 mM and 1 mM re
duced IL-8 binding ability and IL-8-induced migration of the cells by
45% and 65%, respectively. Dithiothreitol could regenerate the binding
capacity and the ligand could protect the receptor from the effect of
the reagent. All the evidence suggests that one or more critical thio
l residues are located in the IL-8 binding site of the receptor which
are indispensible for normal functions of IL-8.