An isocratic anion exchange, high performance liquid chromatography (H
PLC) method was developed to analyze for five of the six vitamers of B
-6. Recoveries of the B6 vitamers in a standard were 100% to 97%. Reco
veries in fried chicken breast ranged from 96% for pyridoxal phosphate
to 102% for pyridoxine (PN). Recoveries in raw chicken breast ranged
from 86% for pyridoxamine to 102% for PN. Processing chicken by frying
reduced the total vitamer by 6.5%. Vitamers of B-6 were relatively st
able to frying.