VITAMIN-D STABILITY IN MILK

A method was developed to determine vitamin D3 in milk. It includes sa ponification, solid phase extraction and HPLC. Recovery of added vitam in D3 was 93%. Vitamin D3 concentrations in commercial milks were vari able. Stability studies showed that on exposure to light, there was a slight loss of vitamin D3 from fortified milk. Air exposure did not af fect stability in milk. Upon standing there was some stratification of the vitamin in milk containers with slightly more vitamin D3 in the t op layer of milk than at the bottom.