A. Abdollahi et al., TOCOPHEROL MICRO-EXTRACTION METHOD WITH APPLICATION TO QUANTITATIVE-ANALYSIS OF LIPOPHILIC NUTRIENTS, Journal of food science, 58(3), 1993, pp. 663-666
A method proposed for determining vitamin E consists of extraction wit
h n-hexane/2-propanol and separation by normal-phase HPLC. It was used
to monitor tocopherol content of eggs metabolically enriched with vit
amin E. It accurately evaluated nutritional uniformity of many samples
in a relatively short time. Chromatographic resolution showed alpha-t
ocopherol as the only major peak in the enriched eggs. Quantification
with HPLC or GC demonstrated that the extraction technique could also
be used to determine cholesterol and fatty acids. Results from eggs co
ntaining varied ratios of fatty acids indicate that the method did not
differentially extract different types of fatty acids.