A NEW APPROACH TO THE IMMUNOCYTOCHEMISTRY OF CAMP - INITIAL CHARACTERIZATION OF ANTIBODIES AGAINST ACROLEIN-FIXED CAMP

Authors
DEVENTE J SCHIPPER J STEINBUSCH HWM
Citation
J. Devente et al., A NEW APPROACH TO THE IMMUNOCYTOCHEMISTRY OF CAMP - INITIAL CHARACTERIZATION OF ANTIBODIES AGAINST ACROLEIN-FIXED CAMP, Histochemistry, 99(6), 1993, pp. 457-462
Citations number
26
Categorie Soggetti
Cytology & Histology
Journal title
Histochemistry → ACNP
ISSN journal
03015564
Volume
99
Issue
6
Year of publication
1993
Pages
457 - 462
Database
ISI
SICI code
0301-5564(1993)99:6<457:ANATTI>2.0.ZU;2-8
Abstract
A method is described to couple cyclic adenosine 3',5' monophosphate ( cAMP) to a carrier protein by means of acrolein. Antibodies against th is conjugate were raised in mice. These antibodies proved to be highly specific for acrolein-fixed cAMP in a gelatin model system. Slices (3 00 mum in thickness) from rat cerebral cortex were incubated in vitro and the dopaminergic control of adenylate cyclase activity was drug-ma nipulated. This manipulation was visualized by application of the cAMP -antisera on cryostat sections of the acrolein fixed slices.