G. Bein et al., A LONGITUDINAL PROSPECTIVE-STUDY OF CYTOMEGALOVIRUS-PP65 ANTIGENEMIA IN RENAL-TRANSPLANT RECIPIENTS, Transplant international, 6(4), 1993, pp. 185-190
Cytomegalovirus (CMV)-encoded pp65 antigen in peripheral blood leukocy
tes (CMV antigenemia) was investigated in 1017 serial samples from 64
patients for 16 weeks after renal transplantation in a prospective stu
dy. In 110 samples from 24 patients, at least one antigen-positive leu
kocyte was identified. The median number of stained cells was 4 (range
1-1000) per 4 x 10(5) leukocytes. Twenty-one of 24 patients with sero
logical signs of an active CMV infection were antigen-positive (sensit
ivity 87.5 %), whereas 3 patients with antigenemia did not show serolo
gical signs of infection during the observation period (specificity 92
.5 %). Positive results were obtained 19 days (median) before serologi
cal response and 9 days (median) before the onset of CMV syndrome. The
sensitivity in defining a CMV syndrome was 100 % (n = 8). In all pati
ents who presented with CMV syndrome, antigenemia was present prior to
the onset of symptoms or on the same day. In contrast, serological mo
nitoring rendered the diagnosis of CMV infection possible at the onset
of clinical symptoms in only two of eight patients. We conclude that
(1) insufficient results obtained with the CMV antigenemia assay by ot
her investigators are mainly due to technical problems that can easily
be overcome by the protocol presented and (2) the detection of CMV pp
65 antigen in peripheral blood leukocytes is an excellent tool for rap
id and early diagnosis of CMV infection.