A LONGITUDINAL PROSPECTIVE-STUDY OF CYTOMEGALOVIRUS-PP65 ANTIGENEMIA IN RENAL-TRANSPLANT RECIPIENTS

Citation
G. Bein et al., A LONGITUDINAL PROSPECTIVE-STUDY OF CYTOMEGALOVIRUS-PP65 ANTIGENEMIA IN RENAL-TRANSPLANT RECIPIENTS, Transplant international, 6(4), 1993, pp. 185-190
Citations number
NO
Categorie Soggetti
Surgery
Journal title
ISSN journal
09340874
Volume
6
Issue
4
Year of publication
1993
Pages
185 - 190
Database
ISI
SICI code
0934-0874(1993)6:4<185:ALPOCA>2.0.ZU;2-4
Abstract
Cytomegalovirus (CMV)-encoded pp65 antigen in peripheral blood leukocy tes (CMV antigenemia) was investigated in 1017 serial samples from 64 patients for 16 weeks after renal transplantation in a prospective stu dy. In 110 samples from 24 patients, at least one antigen-positive leu kocyte was identified. The median number of stained cells was 4 (range 1-1000) per 4 x 10(5) leukocytes. Twenty-one of 24 patients with sero logical signs of an active CMV infection were antigen-positive (sensit ivity 87.5 %), whereas 3 patients with antigenemia did not show serolo gical signs of infection during the observation period (specificity 92 .5 %). Positive results were obtained 19 days (median) before serologi cal response and 9 days (median) before the onset of CMV syndrome. The sensitivity in defining a CMV syndrome was 100 % (n = 8). In all pati ents who presented with CMV syndrome, antigenemia was present prior to the onset of symptoms or on the same day. In contrast, serological mo nitoring rendered the diagnosis of CMV infection possible at the onset of clinical symptoms in only two of eight patients. We conclude that (1) insufficient results obtained with the CMV antigenemia assay by ot her investigators are mainly due to technical problems that can easily be overcome by the protocol presented and (2) the detection of CMV pp 65 antigen in peripheral blood leukocytes is an excellent tool for rap id and early diagnosis of CMV infection.