INJURY TO CULTURED LIVER ENDOTHELIAL-CELLS DURING COLD PRESERVATION -ENERGY-DEPENDENT VERSUS ENERGY-DEFICIENCY INJURY

Previously, we demonstrated an energy-dependent injury to cultured liv er endothelial cells during cold incubation in University of Wisconsin (UW) solution. Here, the effects of Histidine-Tryptophan-Ketoglutarat e (HTK) and Euro-Collins (EC) solutions on these cells were studied. I n HTK solution, 83% +/- 4% of the cells had lost viability after 9 h o f incubation at 4-degrees-C. The addition of cyanide (1 mM) to simulat e hypoxic conditions protected the cells to the extent that only 9% +/ - 1% of the cells lost viability over the same period; the addition of glucose (10 mM) led to increased cell injury. ATP levels were highest in the incubations with the most rapid loss of viability. In Krebs-He nseleit buffer and EC solution, in contrast, cell injury increased upo n addition of cyanide; the addition of glucose to Krebs-Henseleit buff er decreased injury. We conclude that the injury to cultured liver end othelial cells during cold incubation in HTK solution is energy-depend ent, as it is in UW solution, whereas cells behave differently in EC s olution and Krebs-Henseleit buffer.