PROTECTIVE EFFECT OF THE PAF ANTAGONIST BN-52021 IN AN EXPERIMENTAL RENAL WARM ISCHEMIA MODEL

Platelet activating factor is involved in warm ischemic damage. We stu died the effect of the PAF receptor antagonist BN 52021 in an experime ntal model of 60 min of renal warm ischemia in which the left kidney w as flushed with Euro-Collins solution and a right nephrectomy was perf ormed. Eighty Wistar rats were divided into a sham-operated group, two control groups, and four study groups, according to the dosage and ro ute of BN 52021 administration. BN 52021 was used in the flush solutio n at concentrations of 0.1 and 0.5 mg/ml, or intravenously prior to is chemia at 5 and 10 mg/kg body weight. Creatinine clearance per 100 g b ody weight, fractional sodium excretion, and conventional histology we re studied. Rats that received BN 52021 intravenously showed a signifi cantly higher creatinine clearance than controls. Intravenous BN 52021 produced a higher acceleration of renal function recovery at 10 mg/kg than at 5 mg/kg body weight. Conventional histology was better in ani mals that received BN 52021 at 10 mg/kg body weight than in controls. Addition of BN 52021 to Euro-Collins flushing solution showed no prote ctive effect. We conclude that intravenous BN 52021 shows a renal prot ective effect against warm ischemia.