TRANSFERRING GUS GENE INTO INTACT RICE CELLS BY LOW-ENERGY ION-BEAM

Authors
YU ZL YANG JB WU YJ CHENG BJ HE JJ HUO YP
Citation
Zl. Yu et al., TRANSFERRING GUS GENE INTO INTACT RICE CELLS BY LOW-ENERGY ION-BEAM, Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms, 80-1, 1993, pp. 1328-1331
Citations number
3
Categorie Soggetti
Physics, Nuclear","Nuclear Sciences & Tecnology","Instument & Instrumentation
Journal title
Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms → ACNP
ISSN journal
0168583X
Volume
80-1
Year of publication
1993
Part
2
Pages
1328 - 1331
Database
ISI
SICI code
0168-583X(1993)80-1:<1328:TGGIIR>2.0.ZU;2-1
Abstract
A new technique of transferring genes by low energy ion beam has been reported in this paper. The Gus and CAT (chloramphenicol acetyltransfe rase) genes, as ''foreign'' genetic materials, were introduced into th e suspension cells and ripe embryos of rice by implantation of 20-30 k eV Ar+ at doses ranging from 1 x 10(15) to 4 x 10(15) ions/cm2. The ac tivities of CAT and Gus were detected in the cells and embryos after s everal weeks. The results indicate that the transfer was a success.