FAILURE OF APPLICATION OF A TURBIDIMETRIC METHOD FOR THE DETERMINATION OF CHORIOLYTIC ACTIVITY OF COREGONINAE HATCHING LIQUID

An attempt was undertaken to assess Coregoninae hatching liquid activi ty (choriolytic activity) towards its natural substrate - chorion. Wit hin the whole incubation period the reaction mixture showed no plausib le changes in turbidity which resulted in a failure of turbidimetry fo r determining choriolytic activity of Coregoninae hatching enzyme. Res ults of the study may be explained by any of the reason(-s) listed bel ow: 1: hatching enzyme of Coregoninae digests chorion in a different w ay than does medaka (Oryzias latipes) chorionase, 2: homogenization pr ocess could cause some denaturative changes in the inner protein layer of chorion in Coregoninae, 3: chorionase turnover was hindered due to competitive binding of some compound(s) of the reaction medium to the catalytic site on its molecule, resulting in gradual inactivation of the hatching enzyme.