PREPARATION AND USE OF A MONOCLONAL-ANTIBODY TO DETECT CHLAMYDIA-PSITTACI ANTIGEN IN PARAFFIN-EMBEDDED TISSUE-SECTIONS

Authors
BOLLO E BIOLATTI B DONN A TURILLI C WILSMORE AJ
Citation
E. Bollo et al., PREPARATION AND USE OF A MONOCLONAL-ANTIBODY TO DETECT CHLAMYDIA-PSITTACI ANTIGEN IN PARAFFIN-EMBEDDED TISSUE-SECTIONS, Research in Veterinary Science, 53(3), 1992, pp. 393-396
Citations number
18
Categorie Soggetti
Veterinary Sciences
Journal title
Research in Veterinary Science → ACNP
ISSN journal
00345288
Volume
53
Issue
3
Year of publication
1992
Pages
393 - 396
Database
ISI
SICI code
0034-5288(1992)53:3<393:PAUOAM>2.0.ZU;2-4
Abstract
A murine monoclonal antibody prepared against an ovine abortion isolat e of Chlamydia psittaci (A22/Teramo) revealed specific binding to a 57 kDa chlamydial antigen in immunoblotting studies. The monoclonal anti body was able to detect intracytoplasmic chlamydial inclusions and sca ttered elementary bodies in infected McCoy cell culture, and on formal in-fixed paraffin-embedded tissue sections both from experimentally in fected mice and from fetal membranes of cases of ovine enzootic aborti on.