EVIDENCE OF A LINK BETWEEN ERYTHROCYTE BAND-3 PHOSPHORYLATION AND ANION TRANSPORT IN PATIENTS WITH IDIOPATHIC CALCIUM-OXALATE NEPHROLITHIASIS

This study was carried out to verify the hypothesis of a link between faster endogenous phosphorylation of band 3 protein, the anion carrier , and anomalous oxalate transmembrane self-exchange found in erythrocy te from calcium oxalate renal stone formers. Agents able to modify P-3 2-labelling of band 3 protein induced a concurrent modification in oxa late transmembrane flux. Cyclic AMP- and phospholipid-sensitive Ca2+-i ndependent protein kinases seem to be critical modulators of band 3 fu nction. These observations demonstrate a close link between the band 3 phosphorylation state and its anion transport function, and provide n ew insights into the pathogenetic mechanisms of the cellular anomalies observed in calcium-oxalate renal stone disease.