DETAILED GENETIC AND PHYSICAL MAP OF THE 3P-CHROMOSOME REGION SURROUNDING THE FAMILIAL RENAL-CELL CARCINOMA CHROMOSOME-TRANSLOCATION, T(3,8)(P14.2,Q24.1)

Extensive studies of loss of heterozygosity of 3p markers in renal cel l carcinomas (RCCs) have established that there are at least three reg ions critical in kidney tumorigenesis, one most likely coincident with the von Hippel-Lindau gene at 3p25.3, one in 3p21 which may also be c ritical in small cell lung carcinomas, and one in 3p13-p14.2, a region which includes the 3p chromosome translocation break of familial RCC with the t(3;8)-(p14.2;q24.1) translocation. A panel of rodent-human h ybrids carrying portions of 3p, including a hybrid carrying the deriva tive 8 (der(8)(8pter-->8q24.1=3p14.2-->3pter)) from the RCC family, ha ve been characterized using 3p anchor probes and cytogenetic methods. This 3p panel was then used to map a large number of genetically mappe d probes into seven physical intervals between 3p12 and 3pter defined by the hybrid panel. Markers have been physically, and some geneticall y, placed relative to the t(3;8) break, such that positional cloning o f the break is feasible.