ELECTROPHORETIC PROTEIN-ANALYSIS FOR THE IDENTIFICATION OF DOUBLED HAPLOID 1A-1R, 1B-1R WHEAT-RYE DOUBLE TRANSLOCATION LINES AND FOR THE ASSESSMENT OF THEIR GENETIC STABILITY

Eighteen available doubled haploid wheat lines with a cytologically pr oven 1A-1R, 1B-1R double translocation, which where derived via anther culture from four crosses of the 1A-1R wheat-rye translocation cv ''A migo'' with several 1B-1R wheat-rye translocation forms, were subjecte d to electrophoretic seed protein analysis. Besides, the five parents used in the crosses and some other wheat cultivars and doubled haploid lines (19 with a 1B-1R single translocation, 10 with a 1A-1R transloc ation and 7 without any 1R translocation) were also included in the in vestigation. It was found that the gliadin patterns visualized after S DS polyacrylamide gel electrophoresis of alcohol-soluble seed protein extracts can differentiate not only 1B-1R and 1A-1R translocation form s from wheats without any 1R-translocation chromosome, but also 1B-1R and 1A-1R wheats from each other. Moreover, 1A-1R, 1B-1R double transl ocation lines can be distinguished as well due to characteristic diffe rences revealed between 1A-1R and 1B-1R translocation forms. Thus, all of tested dh1- and dh2-grains of the double translocation lines showe d the expected doublet: the 1A-1R translocation (''Amigo'')-typical ry e band and the 1B-1R translocation (''Kawkas'')-typical rye band. Cons equently, gliadin patterns estimated after SDS electrophoresis may be used as markers for the fast detection of the desired 1A-1R, 1B-1R dou ble translocation forms among 1A-1R single translocation lines, 1B-1R single translocation lines and lines without any 1R-translocation in t he progenies of appropriate crosses. Furthermore, by means of gliadin tests on the dh2-generation the excellent stability of the double tran slocation 1A-1R, 1B-1R during more than one propagation phase has been proven. Estimations of high-molecular weight (HMW) glutenin subunits coded by 1A and 1B chromosomes are compatible with the double transloc ation constitution. A few deviating results can be explained by crossi ng-over events. Seed protein analysis revealed that it is possible to produce 1A-1R, 1B-1R double translocation lines with good glutenin com positions provided that adequate favourable parents are used.