RESERPINE CAUSES DIFFERENTIAL CHANGES IN THE MESSENGER-RNA LEVELS OF CHROMOGRANIN-B, SECRETOGRANIN-II, CARBOXYPEPTIDASE-H, ALPHA-AMIDATING MONOOXYGENASE, THE VESICULAR AMINE TRANSPORTER AND OF SYNAPTIN SYNAPTOPHYSIN IN RAT-BRAIN

Citation
Sk. Mahata et al., RESERPINE CAUSES DIFFERENTIAL CHANGES IN THE MESSENGER-RNA LEVELS OF CHROMOGRANIN-B, SECRETOGRANIN-II, CARBOXYPEPTIDASE-H, ALPHA-AMIDATING MONOOXYGENASE, THE VESICULAR AMINE TRANSPORTER AND OF SYNAPTIN SYNAPTOPHYSIN IN RAT-BRAIN, Molecular brain research, 19(1-2), 1993, pp. 83-92
Citations number
50
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
0169328X
Volume
19
Issue
1-2
Year of publication
1993
Pages
83 - 92
Database
ISI
SICI code
0169-328X(1993)19:1-2<83:RCDCIT>2.0.ZU;2-4
Abstract
Brains of rats treated with a high dose of reserpine were analyzed by in situ hybridization. The mRNA levels of several components of large dense core and small synaptic vesicles were determined. After drug tre atment the secretogranin II message was elevated in the parvocellular neurons of the paraventricular nucleus, in the zona incerta, dorsal ra phe, locus coeruleus and in the nucleus tractus solitarius. The levels of chromogranin B mRNA were increased in the dorsal raphe and in the substantia nigra compacta. In control animals messages for synaptin/sy nptophysin could be found in most of the nuclei investigated, that of the vesicular amine transporter was only detectable in substantia nigr a compacta, the dorsal raphe and the locus coeruleus whereas those of carboxypeptidase H and the alpha-amidating monooxygenase could only be determined in the paraventricular nucleus. All these messages were no t changed after reserpine. We conclude that the chromogranin B/secreto granin II messages are regulated concomitantly with various neuropepti des. They represent useful general markers to identify stimulated neur ons. Our results are consistent with the concept that stimulation of n eurons leads to an increased synthesis of secretory peptides and conse quently to large dense core vesicles filled with higher quanta of pept ides.