RESERPINE CAUSES DIFFERENTIAL CHANGES IN THE MESSENGER-RNA LEVELS OF CHROMOGRANIN-B, SECRETOGRANIN-II, CARBOXYPEPTIDASE-H, ALPHA-AMIDATING MONOOXYGENASE, THE VESICULAR AMINE TRANSPORTER AND OF SYNAPTIN SYNAPTOPHYSIN IN RAT-BRAIN
Sk. Mahata et al., RESERPINE CAUSES DIFFERENTIAL CHANGES IN THE MESSENGER-RNA LEVELS OF CHROMOGRANIN-B, SECRETOGRANIN-II, CARBOXYPEPTIDASE-H, ALPHA-AMIDATING MONOOXYGENASE, THE VESICULAR AMINE TRANSPORTER AND OF SYNAPTIN SYNAPTOPHYSIN IN RAT-BRAIN, Molecular brain research, 19(1-2), 1993, pp. 83-92
Brains of rats treated with a high dose of reserpine were analyzed by
in situ hybridization. The mRNA levels of several components of large
dense core and small synaptic vesicles were determined. After drug tre
atment the secretogranin II message was elevated in the parvocellular
neurons of the paraventricular nucleus, in the zona incerta, dorsal ra
phe, locus coeruleus and in the nucleus tractus solitarius. The levels
of chromogranin B mRNA were increased in the dorsal raphe and in the
substantia nigra compacta. In control animals messages for synaptin/sy
nptophysin could be found in most of the nuclei investigated, that of
the vesicular amine transporter was only detectable in substantia nigr
a compacta, the dorsal raphe and the locus coeruleus whereas those of
carboxypeptidase H and the alpha-amidating monooxygenase could only be
determined in the paraventricular nucleus. All these messages were no
t changed after reserpine. We conclude that the chromogranin B/secreto
granin II messages are regulated concomitantly with various neuropepti
des. They represent useful general markers to identify stimulated neur
ons. Our results are consistent with the concept that stimulation of n
eurons leads to an increased synthesis of secretory peptides and conse
quently to large dense core vesicles filled with higher quanta of pept
ides.