Jg. Daly et Rmw. Stevenson, NUTRIENT-REQUIREMENTS OF RENIBACTERIUM-SALMONINARUM ON AGAR AND IN BROTH MEDIA, Applied and environmental microbiology, 59(7), 1993, pp. 2178-2183
In well-aerated broth cultures, good growth of Renibacterium salnwnina
rum was obtained in a serum-free medium consisting of 1% peptone, 1% y
east extract, and 0.1% L-cysteine (PYC broth). In contrast, serum or c
harcoal is required for growth on agar medium. Charcoal treatment of b
roth media, either before bacterial inoculation or during growth, incr
eased the growth of R. salmoninarum, whereas the surfactants Tween 20
and Tween 80 inhibited growth. L-Cysteine was essential for optimal gr
owth. Other organic sulfur compounds, such as D-cysteine, L-methionine
, homocysteine, homocysteine thiolactone, and reduced glutathione, sup
ported only lower levels of growth, while cystine and dithiothreitol d
id not allow growth.