IN-SITU HYBRIDIZATION MAPPING OF HISTONE GENES IN ANOPHELES-ALBIMANUS

The histone genes of Anopheles albimanus were mapped by in situ hybrid ization to 6 bands in Region 34A on the right arm of chromosome 3. A g enomic library was made by cloning fragments of 15 to 23 kb (derived f rom partial EcoRI digestion) into the phage vector, EMBL4, and probed with the histone gene repeat of Drosophila melanogaster. Thirty-two ph ages containing histone gene sequences were isolated from about 10(5) plaque-forming units (pfu). Complete EcoRI digestion of DNA from 5 of the 32 recombinant phages and the genomic DNA of An. albimanus yielded a single 3.84-kb fragment that contained sequences homologous to the 5 histone genes of D. melanogaster. This 3.84-kb unit of mosquito hist one genes was subcloned into puc19 plasmid, and the resulting clone (p albi34A) was used for in situ hybridization to salivary gland chromoso mes.