ANALYSIS OF WHEY PROTEINS BY CAPILLARY ELECTROPHORESIS USING BUFFER-CONTAINING POLYMERIC ADDITIVES

This paper shows the possibilities of capillary electrophoresis for an alysis of the major whey proteins (beta-lactoglobulin A, beta-lactoglo bulin B, alpha-lactalbumin, and BSA) in standard samples and raw and U HT milks. A new separation buffer, consisting of Tris-boric acid, SDS, and polyethylene glycol 8000, was used for the separation. Good migra tion time reproducibility was achieved for the proteins of standard sa mples and those of real samples analyzed on the same day and on 3 diff erent d. Area reproducibility for the protein peaks ranged from 2.7% f or beta-lactoglobulins to 17.9% for BSA, including standard and real s amples. Also, the concentration of the major proteins determined in th e milks studied agrees quite well with the values obtained by HPLC. Fu rthermore, analysis time can be fourfold smaller using capillary elect rophoresis than that usually obtained in HPLC.