No. Mocchiutti et al., CHRONIC INGESTION OF BROMINATED VEGETABLE-OILS - ITS ACTION ON HEPATIC SECRETION AND CATABOLISM OF PLASMA-LIPOPROTEINS, Archivos latinoamericanos de nutricion, 42(4), 1992, pp. 403-408
We have previously reported that normal Wistar rats fed during 105 day
s with standard laboratory chow, supplemented with 0.5g of brominated
vegetable oil (olive, sunflower) per 100 g of diet showed a significan
t increase of triglyceride and cholesterol content in both heart and l
iver. This was accompanied by a significant decrease of plasma lipid l
evels. Fluctuations in plasma triglyceride concentrations may be a res
ult of either variations in the liver secretion rate of very low densi
ty lipoprotein-triglyceride (VLDL-TG), or changes in their removal rat
e by extrahepatic tissues or both. In the present work we have studied
the contribution of both VLDL-TG secretion, and removal rates of plas
ma TG in the decrease of plasma TG levels, in rats fed during 105 days
with a standard laboratory chow supplemented with 0,5 g per 100 g of
brominated vegetable oil. VLDL-TG secretion was estimated by measuring
the accumulation of plasma TG following the injection of TRITON WR 13
39 and the removal rate of plasma TG by assaying plasma post-heparin l
ipolytic total (PHLA) and hepatic (H-TGL) lipase activities. In additi
on, the major lipid composition of plasma lipoprotein fractions were m
easured. Results were compared to those of a control group fed a labor
atory chow diet during the same period of time. Our results show a dec
rease in both VLDL-TG secretion and plasma TG pool size accompanied by
normal PHLA and H-TGL activities in animals fed the diet supplemented
with brominated oils. However, the proportion of the major lipid comp
onents of the plasma lipoproteins fractions were unchanged. This findi
ng could indicate an impaired synthesis and/or secretion of VLDL-TG li
poprotein by the liver. In summary, the toxicologic effects observed d
uring chronic intake of diets supplemented with brominated oils, sugge
st the need to undertake further biochemical studies of this food addi
tive used in the manufacture of certain citrus flavored beverages.