INHIBITION OF ORNITHINE DECARBOXYLASE ACTIVITY DECREASES POLYAMINES AND SUPPRESSES DNA-SYNTHESIS IN HUMAN COLONIC LAMINA PROPRIA LYMPHOCYTES

Ornithine decarboxylase (ODC) and the polyamines are essential for cel l proliferation in a variety of cells including lymphocytes. In this s tudy, we investigated the potential role of ODC and polyamines in huma n colonic lamina propria lymphocytes (LPL) compared to peripheral bloo d lymphocytes (PBL). Our results show that con A stimulation of LPL an d PBL was associated with marked increases in ODC and polyamines. The specific inhibitor of ODC, alpha-difluoromethylomithine (DFMO), result ed in a complete inhibition of ODC activity and depletion of putrescin e, spermidine and spermine levels. DFMO also suppressed DNA synthesis of LPL and PBL by up to 48% and 62% respectively. This antiproliferati ve effect was reversed by adding back the polyamines putrescine (10 mu M), spermidine (10 muM) or spermine (10 muM) to the culture medium. We conclude that ODC and the polyamines are important for human LPL prol iferation, and hence may play a role in human mucosal immune function.