LOCALIZED DETECTION OF GLIOMA GLYCOLYSIS USING EDITED H-1 MRS

In vivo H-1 MRS can be used to detect and quantify the lactate resonan ce at 1.3 ppm provided that overlapping lipid resonances are eliminate d. A homonuclear spectral editing method was developed to acquire unco ntaminated H-1 spectra of lactate with adiabatic pulses. An advantage of the adiabatic pulse sequence is the ability to induce uniform flip angles and to maximize sensitivity in applications employing surface c oil transmitters which produce highly inhomogeneous B1. Glycolytic act ivity in an intracerebral C6 glioma in rats was monitored by using adi abatic editing sequences to observe [3-C-13]lactate produced from infu sed [1-C-13]glucose. Acute hyperglycemia (serum glucose >22 mM, n = 10 ) had no significant effect (P = 0.08) on the total ([C-12] + [C-13]) tumor lactate signal intensity.