CLONING AND CHARACTERIZATION OF A BOVINE ALPHA-INTERFERON RECEPTOR

Authors
LIM JK LANGER JA
Citation
Jk. Lim et Ja. Langer, CLONING AND CHARACTERIZATION OF A BOVINE ALPHA-INTERFERON RECEPTOR, Biochimica et biophysica acta, 1173(3), 1993, pp. 314-319
Citations number
41
Categorie Soggetti
Biophysics,Biology
Journal title
Biochimica et biophysica acta → ACNP
ISSN journal
00063002
Volume
1173
Issue
3
Year of publication
1993
Pages
314 - 319
Database
ISI
SICI code
0006-3002(1993)1173:3<314:CACOAB>2.0.ZU;2-2
Abstract
A bovine interferon a receptor (BoIFN-alphaR1) cDNA, homologous to the human cDNA [1], was isolated. Transfection of the BoIFN-alphaR1 cDNA into monkey COS cells results in a large increase in high-affinity bin ding sites for human IFN-alphaA and IFN-alphaB. Covalent crosslinking of radiolabeled HuIFN-alphaA and -alphaB demonstrates that the complex of [P-32]HuIFN with the BoIFN-alphaR1 protein (predicted mass, 61375) expressed in COS cells migrates as a 140-150 kDa band.