MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF THE MONKEY AND HUMAN TISSUE KALLIKREIN GENES

Cynomolgus monkey renal kallikrein cDNA and genomic human tissue kalli krein gene were cloned. The monkey gene encodes a 257 amino acid (aa) preprokallikrein and exhibits 95% and 92% homology to the human at nuc leotide (nt) and aa level, respectively. The monkey gene encodes a 233 -aa mature kallikrein versus a 238-aa in human. The human kallikrein g ene and urinary kallikrein both contain a Lys-162 instead of the repor ted Glu-162. Human, monkey and rat renal/pancreatic kallikrein genes e volve with a N-glycosylation containing domain (aa 81-87) which is abs ent in porcine and is non-glycosylable in mice. Only human kallikrein evolves with an additional Thr-108 and with a N-glycosylation site at aa-141.