BIOSYNTHESIS OF RIBOFLAVIN - CLONING, SEQUENCING, MAPPING, AND EXPRESSION OF THE GENE CODING FOR GTP CYCLOHYDROLASE-II IN ESCHERICHIA-COLI

Citation
G. Richter et al., BIOSYNTHESIS OF RIBOFLAVIN - CLONING, SEQUENCING, MAPPING, AND EXPRESSION OF THE GENE CODING FOR GTP CYCLOHYDROLASE-II IN ESCHERICHIA-COLI, Journal of bacteriology, 175(13), 1993, pp. 4045-4051
Citations number
30
Categorie Soggetti
Microbiology
Journal title
ISSN journal
00219193
Volume
175
Issue
13
Year of publication
1993
Pages
4045 - 4051
Database
ISI
SICI code
0021-9193(1993)175:13<4045:BOR-CS>2.0.ZU;2-D
Abstract
GTP cyclohydrolase II catalyzes the first committed step in the biosyn thesis of riboflavin. The gene coding for this enzyme in Escherichia c oli has been cloned by marker rescue. Sequencing indicated an open rea ding frame of 588 bp coding for a 21.8-kDa peptide of 196 amino acids. The gene was mapped to a position at 28.2 min on the E. coli chromoso me and is identical with ribA. GTP cyclohydrolase II was overexpressed in a recombinant strain carrying a plasmid with the cloned gene. The enzyme was purified to homogeneity from the recombinant strain. The N- terminal sequence determined by Edman degradation was identical to the predicted sequence. The sequence is homologous to the 3' part of the central open reading frame in the riboflavin operon of Bacillus subtil is.