MOLECULAR-GENETIC APPROACHES TO THE ANALYSIS OF NEUROPEPTIDE RECEPTORS

Within the last few years, an increasing number of neuropeptide recept ors have been structurally and functionally characterized. The analysi s of these receptors, along with a precise understanding of their phar macology and signal transduction pathways, has been facilitated by the use of molecular genetic techniques. This review will highlight some of the techniques currently being used to study neuropeptide receptors . Methods for isolation of complementary DNA clones for receptors, ana lysis of receptor messenger RNA expression and characterization of clo ned receptors expressed in cultured cells are reviewed. In addition, a genetic approach for the analysis of neuropeptide receptor function i n the context of a whole mammalian organism is discussed.