Cm. Mansbach et Rf. Dowell, PORTAL TRANSPORT OF LONG ACYL-CHAIN LIPIDS - EFFECT OF PHOSPHATIDYLCHOLINE AND LOW INFUSION RATES, The American journal of physiology, 264(6), 1993, pp. 1082-1089
The transport of absorbed long acyl chain lipids in the portal vein of
rats has been shown to be 39% when the duodenal input rate is 135 mum
ol/h glyceryl trioleate (TO) [C. M. Mansbach II, R. F. Dowell, and D.
Pritchett, Am. J. Physiol. 255 (Gastrointest. Liver Physiol. 18): G530
-G539, 1991]. These calculations were based on a new experimental mode
l in which portal flux is calculated from the knowledge of portal flow
and the concentration of the lipids in excess in the portal vein vs.
the carotid artery. To test this model, rats were infused for 6 h with
a low rate of [H-3]TO (27 mumol/h) with or without phosphatidylcholin
e (9 mumol/h) or with [H-3]TO (135 mumol/h) plus phosphatidylcholine (
9 mumol/h). In all three cases, portal flux was expected to be less. P
ortal transport was 16.5% of the input rate in the low-dose group, 1.4
% in the high-dose group given phosphatidylcholine, and 0.5% in the lo
w-dose plus phosphatidylcholine group. There was no net transport of f
atty acid in the portal vein in any of the three cases. These data sho
w that portal lipid transport is dependent on the lipid load and that
it is greatly reduced at high loads by including phosphatidylcholine i
n the lipid infusion.