IMMOBILIZED RHODANESE - SOME ASPECTS OF ANION INHIBITION-KINETICS ANDMODULATION BY CATIONS

Authors
NOK AJ NASIR SM SAADATU Y
Citation
Aj. Nok et al., IMMOBILIZED RHODANESE - SOME ASPECTS OF ANION INHIBITION-KINETICS ANDMODULATION BY CATIONS, Journal of biochemical toxicology, 8(2), 1993, pp. 57-62
Citations number
14
Categorie Soggetti
Biology
Journal title
Journal of biochemical toxicology → ACNP
ISSN journal
08872082
Volume
8
Issue
2
Year of publication
1993
Pages
57 - 62
Database
ISI
SICI code
0887-2082(1993)8:2<57:IR-SAO>2.0.ZU;2-L
Abstract
The rat liver rhodanese (thiosulphate: cyanide sulfurtransferase EC 2. 6.1.1) has been immobilized on polyacrylamide gels. The immobilized en zyme had a pH optimum of 7.4 and Km values of 3.25 mM and 1.12 mM for S2O32- and KCN, respectively. The enzyme was competitively inhibited b y NaNO2 and CH3COONa and noncompetitively by amyl-nitrite. A modulatio n of activity was observed in the presence of Ca2+, Zn2+, and Cu2+. Th e results are discussed in line with the detoxicating function of live r rhodanese.