A. Ohno et al., EFFECT OF TEMPERATURE-CHANGE AND AERATION ON THE PRODUCTION OF THE ANTIFUNGAL PEPTIDE ANTIBIOTIC ITURIN BY BACILLUS-SUBTILIS NB22 IN LIQUIDCULTIVATION, Journal of fermentation and bioengineering, 75(6), 1993, pp. 463-465
The effects of temperature and air supply on the production of the ant
ifungal lipopeptide antibiotic, iturin by Bacillus subtilis NB22 in a
5-d course of liquid cultivation were investigated on a flask scale. C
hanging the temperature from 30-degrees-C to 25, 37, 42, or 48-degrees
-C for one day during the 5-d cultivation reduced the amount of iturin
producted in comparison with cultivation at a constant 30-degrees-C.
The temperature on the first day was notably critical. When the air su
pply was varied by changing the pings of the shaking flasks, the air s
upply was also critical on the first day. When cells spent the first d
ay with an inappropriate temperature or air supply, the decrease in it
urin production could not be compensated for during the subsequent 4-d
cultivation at an adequate temperature and air supply. Although ituri
n production was significant on the second and third days when the gro
wth of the bacterium had leveled off, the initial conditions severely
affected the yield of the product.