NOURSEOTHRICIN (STREPTOTHRICIN) INACTIVATED BY A PLASMID PIE636 ENCODED ACETYL TRANSFERASE OF ESCHERICHIA-COLI - LOCATION OF THE ACETYL GROUP

Authors
ZAHRINGER U VOIGT W SELTMANN G
Citation
U. Zahringer et al., NOURSEOTHRICIN (STREPTOTHRICIN) INACTIVATED BY A PLASMID PIE636 ENCODED ACETYL TRANSFERASE OF ESCHERICHIA-COLI - LOCATION OF THE ACETYL GROUP, FEMS microbiology letters, 110(3), 1993, pp. 331-334
Citations number
8
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
110
Issue
3
Year of publication
1993
Pages
331 - 334
Database
ISI
SICI code
0378-1097(1993)110:3<331:N(IBAP>2.0.ZU;2-8
Abstract
Escherichia coli strains harbouring the plasmid pIE636 are able to syn thesize acetylcoenzyme A: streptothricin acetyltransferase (ACSAT). Th e (enzymatic) N-acetylation of streptothricin F is known to contribute significantly towards the loss of antibacterial activity. C-13-NMR an alysis of [C-14]N-acetyl-labelled streptothricin F, produced by ACSAT- catalysed acetylation of streptothricin F and subsequent purification by various chromatographical steps, unequivocally revealed streptothri cin F to be acetylated at the beta-amino group (C16) (and not at the e psilon-amino group (C19)).