HAPLOTYPE ANALYSIS DEFINES A MINIMAL INTERVAL FOR THE MULTIPLE ENDOCRINE NEOPLASIA TYPE-1 (MEN1) GENE

Multiple endocrine neoplasia type 1 (MEN1) is an inherited syndrome ch aracterized by development of multiple endocrine tumors in affected in dividuals, The gene responsible for the disease has been mapped to chr omosome 11q13 by linkage analysis, but the gene itself has not yet bee n identified. We allelotyped 33 affected individuals from an extensive MEN1 kindred using eight polymorphic markers located on chromosome 11 q13, including two new markers (D11S4907 and D11S4908) that we derived and mapped to the SEA-D11S913 region. Analysis of affected individual s revealed two separate recombination events, providing new centromeri c and telomeric boundaries for the MEN1 gene. The present data indicat e the MEN1 gene is located between markers D11S1883 and D11S4907, an a pproximate 2 Mb region on chromosome 11q13.