Gene transfer technologies have facilitated researchers to produce bot
h monocot and dicot transgenic plants, independent of their genotype.
The analysis of molecular markers can accomplish the characterization,
gene mapping and identification, certification and patent protection
of cultivars. The expression of a target gene can be inhibited with an
tisense RNA. With the specific gene transfer, many goals-biological co
ntrol of insect pests and fungi, male sterility, virus resistance, imp
roving seed protein, transgenic plants as 'bioreactors'-can be accompl
ished. Yet more concerted efforts are needed to study the genes relate
d to environmental stress including greenhouse effect, quantitative tr
aits, and field evaluation of transgenes in transgenic plants. The cos
t effectiveness in producing transgenic cultivars and parameters such
as yield, quality, and nutrition will determine the wider use of trans
genic germplasm in plant breeding.