Es. Snigirevskaya et Jj. Komissarchik, A NOVEL TYPE OF MICROTUBULES IN THE FROG URINARY-BLADDER EPITHELIUM STIMULATED BY VASOPRESSIN, Journal of submicroscopic cytology and pathology, 25(3), 1993, pp. 389-396
Changes in the cytoskeleton of granular cells of the frog urinary blad
der under conditions of low and vasopressin-induced water permeability
were investigated. Using a conventional electron microscopical prepar
ation with glutaraldehyde/osmium fixation we found only the usual type
of microtubules in granular cells of both resting and stimulated blad
ders. These microtubules, 20 nm in diameter, were associated with cent
rioles and Golgi complexes. Under the high water permeability conditio
ns the large vacuoles appear in granular cells. The 20-nm microtubules
and microfilaments were connected to the membrane of these vacuoles.
The second type of 'thick' microtubules was found in granular cells of
the stimulated bladder after the fixation with glutaraldehyde without
osmium postfixation or freeze-substitution. The tubulin nature of the
se 'thick' microtubules was identified immunocytochemically. In additi
on, tubulin was present in the granules with crystalline content.