BIOLOGICAL PROPERTIES OF CHLOROFORM METHANOL EXTRACTS OF COXIELLA-BURNETII

Biological properties of antigens of whole cells of C. burnetii in pha se I (WCI), their residue (CMR) and extract (CME) obtained by extracti on of C. burnetii with chloroform/methanol mixture were subjected to a comparative study. It was shown that CME is able to react specificall y with sera containing antibodies against WCI. The inoculation of anim als with CME, but not with WCI and CMR does not cause changes of humor al and cell immunity to Coxiellae and does not strengthen nonspecific antibacterial activity, but it can stimulate the formation of antibodi es. Morphological changes of internal organs of mice were most express ed after the CMR inoculation and were practically absent after the CME inoculation.