C. Zandotti et al., A RAPID DNA EXTRACTION METHOD FROM CULTURE AND CLINICAL-SAMPLES - SUITABLE FOR THE DETECTION OF HUMAN CYTOMEGALOVIRUS BY THE POLYMERASE CHAIN-REACTION, Acta virologica, 37(1), 1993, pp. 106-108
We propose an one-step DNA extraction method suitable for the polymera
se chain reaction. This procedure utilizes Chelex 100, a chelating in
exchange resin. This technique was compared with a traditional techniq
ue (proteinase K lysis, phenol-chloroform extraction and ethanol preci
pitation) for isolation of human cytomegalovirus DNA from clinical sam
ples. The procedure using Chelex 100 appeared to be a simple and fast
extraction method for human cytomegalovirus DNA.