A RAPID DNA EXTRACTION METHOD FROM CULTURE AND CLINICAL-SAMPLES - SUITABLE FOR THE DETECTION OF HUMAN CYTOMEGALOVIRUS BY THE POLYMERASE CHAIN-REACTION

Authors
ZANDOTTI C DELAMBALLERIE X GUIGNOLEVIGNOLI C BOLLET C DEMICCO P
Citation
C. Zandotti et al., A RAPID DNA EXTRACTION METHOD FROM CULTURE AND CLINICAL-SAMPLES - SUITABLE FOR THE DETECTION OF HUMAN CYTOMEGALOVIRUS BY THE POLYMERASE CHAIN-REACTION, Acta virologica, 37(1), 1993, pp. 106-108
Citations number
4
Categorie Soggetti
Virology
Journal title
Acta virologica → ACNP
ISSN journal
0001723X
Volume
37
Issue
1
Year of publication
1993
Pages
106 - 108
Database
ISI
SICI code
0001-723X(1993)37:1<106:ARDEMF>2.0.ZU;2-A
Abstract
We propose an one-step DNA extraction method suitable for the polymera se chain reaction. This procedure utilizes Chelex 100, a chelating in exchange resin. This technique was compared with a traditional techniq ue (proteinase K lysis, phenol-chloroform extraction and ethanol preci pitation) for isolation of human cytomegalovirus DNA from clinical sam ples. The procedure using Chelex 100 appeared to be a simple and fast extraction method for human cytomegalovirus DNA.