The dissolved oxygen content in the Candida utilis chemostat culture m
edium changed, accompanied by changes in affinity values for ethanol a
nd oxygen. The lowest affinities for ethanol and the highest for oxyge
n were noted when oxygen was strictly limited. When carbon and energy
served as the limiting factors, maximum cell. affinity was for ethanol
, and minimum, for oxygen. When dissolved oxygen as the limiting facto
r was increased, the carbon-containing substrate consumption rate was
increased for maintenance of viable cells (m).