Mw. Farnham et Bv. Nelson, UTILITY OF IN-VITRO PROPAGATION FOR FIELD-GROWN BROCCOLI - EFFECT OF GENOTYPE AND GROWING-SEASON, HortScience, 28(6), 1993, pp. 655-656
We examined an in vitro culture method for propagating unconditioned,
field-grown broccoli (Brassica oleracea L. Botrytis group) from pedunc
le explants by testing 20 cultivars in fall and spring. Propagation wa
s affected significantly by genotype (cultivar) and season. The percen
tage of explants regenerating shoots was significantly higher for cult
ivars grown in spring (17% to 100%) than in fall (0% to 66%). Shoot re
generation from explants of plants within a cultivar also varied signi
ficantly (0% to 100%). Additionally the number of propagules produced
per explant was influenced by cultivar and was highly correlated with
the percentage of explants regenerating shoots. This method for propag
ating field-grown broccoli lines is useful, but its applicability can
be limited by genetic and environmental factors.