N. Kapel et al., DETERMINATION OF ANTI-CRYPTOSPORIDIUM COPROANTIBODIES BY TIME-RESOLVED IMMUNOFLUOROMETRIC ASSAY, Transactions of the Royal Society of Tropical Medicine and Hygiene, 87(3), 1993, pp. 330-332
Citations number
16
Categorie Soggetti
Public, Environmental & Occupation Heath","Tropical Medicine
The role of the mucosal immune response against Cryptosporidium has be
en suggested by studies on the therapeutic effects of hyperimmune colo
strum. In order to study the intestinal response to this infection, we
have developed a sandwich-type time-resolved immunofluorometric assay
for the determination of anti-Cryptosporidium coproantibodies. This a
ssay has the inherent sensitivity of an immunoassay without the proble
ms due to background responses from other biological compounds, and is
thus suitable for faecal samples. The intra-assay coefficients of var
iation (5.1%, 4.6%, and 5.8% for immunoglobulins (Ig) A, M and G respe
ctively), inter-assay coefficients of variation (9.4%; 10.5% and 12.2%
for IgA, IgM and IgG, respectively) and specificity (100% for all 3 i
sotypes) were all satisfactory. Using this assay to study 12 patients
with the acquired immune deficiency syndrome (AIDS) who were infected
with cryptosporidiosis, we found a marked elevation of anti-Cryptospor
idium IgA and IgM coproantibody titres relative to 18 healthy control
values, but no correlation with the gravity of the infection in terms
of oocyst shedding. These results suggest that a non-protective mucosa
l immune response develops to Cryptosporidium in AIDS patients.