PERIPHERAL-BLOOD T-CELL EXPANSIONS IN PATIENTS WITH BEHCETS-DISEASE

Behcet's disease (ED) is a chronic multisystemic inflammatory disorder characterized mainly by recurrent oral and genital aphthous ulceratio ns and uveitis. Etiology and pathogenesis of ED remain unknown. T cell receptor (TCR) V alpha/N beta gene product expression as well as J be ta gene segment expression in peripheral blood of BD patients were ana lysed to investigate the possible role of T lymphocytes in the etiopat hogenesis of BD. Flow cytometry with 12 TCR V-specific MoAbs was used for TCRV analyses. J beta gene segment usage by T cell populations exp ressing certain V beta s was determined by polymerase chain reaction ( PCR) technique with V beta- and C beta-specific primers, Southern blot ting of PCR products, and subsequent hybridization with radiolabelled J beta gene segment-specific probes. Although 13 of the 23 ED patients exhibited increases in expression of one or more TCR V-gene products, only expansions among the CD4(+) T cell subset were significantly mor e frequent in ED patients (7/23) compared with healthy controls (0/15) (P = 0.019). Six out of eight cases followed for up to 20 months had at least one expansion correlated with disease activity. A strict pref erence for particular J beta gene segments implicating clonality was a pparent in all analysed T cell expansions and correlated well with dis ease activity. These results suggest a possible involvement of antigen -specific T lymphocytes in the pathogenesis of ED.