Secondary effluent of wastewater treatment plants contains a high numb
er of viruses and other pathogens, which pose a health risk to the pop
ulation, especially when receiving waters are used for bathing and swi
mming, or for growing shellfish. In areas with a high density of popul
ation, where drinking water supply is dependent on surface waters and
contaminated rivers are the primary source of drinking water, failure
of the filtration or of the disinfection step, or of any other ''barri
ers'' supposed to warrant safe potable water, will increase the risk o
f health hazard for the consumer. We have compared the efficiency of v
iral elimination in secondary effluent by flocculation, uv irradiation
and membrane filtration taking naturally occurring, or additionally s
eeded f2 phages, as indicator for viruses. Flocculation decreased the
number of phages present in secondary effluent by more than two logs.
If combined with uv irradiation, the elimination reached five addition
al logs. Membrane filtration eliminated essentially all naturally occu
rring phages. Improvement of the quality of surface waters calls for a
refinement of detection methods for viruses. We have found that the p
olymerase chain reaction (PCR) might be used for detecting viruses in
surface waters.