GENOTYPES AND GENOTYPE-X MEDIUM INTERACTION EFFECTS ON ANDROGENETIC HAPLOID PRODUCTION IN WHEAT (TRITICUM-AESTIVUM L)

The present study investigates the effects of media on the rate of emb ryoid and plantlet formation from anther cultures of four different wi nter wheat cultivars and their F1 hybrids. Anthers from each genotype were cultured on two different media (P2 medium and MN6 medium contain ing either sucrose or maltose). The results showed highly significant differences between the genotypes for all traits studied. The genotype x medium interaction, however, was only significant for responding an thers and embryoid induction, but was not significant in the case of g reen plantlet induction. The best media for embryoid induction and pla ntlet regeneration were the original P2 medium (Ouyang 1986) and 190-2 medium (Zhuang and Xu 1983), respectively. The greatest mean frequenc ies of responding anthers and embryoid induction were observed in the cultivar 'Mv 21' and the transformed values were 7.81 and 19.40 respec tively . Heterosis was observed in the hybrids investigated compared w ith their parents, especially, in the case of plantlet regeneration ab ilities. Therefore using of F1 hybrid plants as anther donors is one o f the most effective methods of increasing the yield of green haploid plants.