AN ORDERED COLLECTION OF BACILLUS-SUBTILIS DNA SEGMENTS CLONED IN YEAST ARTIFICIAL CHROMOSOMES

Authors
AZEVEDO V ALVAREZ E ZUMSTEIN E DAMIANI G SGARAMELLA V EHRLICH SD SERROR P
Citation
V. Azevedo et al., AN ORDERED COLLECTION OF BACILLUS-SUBTILIS DNA SEGMENTS CLONED IN YEAST ARTIFICIAL CHROMOSOMES, Proceedings of the National Academy of Sciences of the United Statesof America, 90(13), 1993, pp. 6047-6051
Citations number
35
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
90
Issue
13
Year of publication
1993
Pages
6047 - 6051
Database
ISI
SICI code
0027-8424(1993)90:13<6047:AOCOBD>2.0.ZU;2-#
Abstract
A collection of 772 Bacillus subtilis DNA segments was obtained by clo ning in yeast artificial chromosomes. The B. subtilis inserts of 288 c lones were mapped by hybridization using as probes 65 cloned genes and 188 isolated insert ends. In this way, 59 inserts were ordered in fou r contigs that cover >98% of the B. subtilis chromosome. This ordered collection is now available for further genetic and physical analysis of the B. subtilis genome.