6 MESSENGER-RNAS WITH DIFFERENT 5' ENDS ARE ENCODED BY A SINGLE GAMMA-GLUTAMYL-TRANSFERASE GENE IN MOUSE

Authors
RAJAGOPALAN S WAN DF HABIB GM SEPULVEDA AR MCLEOD MR LEBOVITZ RM LIEBERMAN MW
Citation
S. Rajagopalan et al., 6 MESSENGER-RNAS WITH DIFFERENT 5' ENDS ARE ENCODED BY A SINGLE GAMMA-GLUTAMYL-TRANSFERASE GENE IN MOUSE, Proceedings of the National Academy of Sciences of the United Statesof America, 90(13), 1993, pp. 6179-6183
Citations number
34
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
90
Issue
13
Year of publication
1993
Pages
6179 - 6183
Database
ISI
SICI code
0027-8424(1993)90:13<6179:6MWD5E>2.0.ZU;2-D
Abstract
The 5' region of the mouse gamma-glutamyltransferase (gammaGT; EC 2.3. 2.2) gene has been cloned and analyzed. This analysis, combined with s equence information obtained from gammaGT cDNA clones, indicates that in mouse a single gammaGT gene codes for six different mRNAs that diff er in their 5' sequences. Analysis of steady-state levels of gammaGT R NA reveals different expression patterns for these RNAs in different o rgans. The six different 5' sequences are widely separated within a 10 -kb region and three of them show 75-86% identity with the three known rat gammaGT cDNAs. Although the heterogeneity of the 5' ends of gamma GT RNAs may be explained in part by alternative splicing, it is likely that multiple promoters are involved in their generation.